Nested polymerase chain reaction for high-sensitivity detection of enteroviral RNA in biological samples
نویسندگان
چکیده
منابع مشابه
Quantitation of enteroviral RNA by competitive polymerase chain reaction.
The polymerase chain reaction (PCR) is a new diagnostic technique for the detection of enteroviral infection; however, it currently provides only qualitative results. The aim of this study was to adapt PCR for the accurate quantitation of enteroviral RNA in clinical specimens. For this purpose, we designed a standard RNA which was homologous to sequences at the 5' end of the coxsackie B3 entero...
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aDivision of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK; blnstituto de Higiene e Medicina Tropical/Centro de Malaria e outras Doen~cas Tropicais, Lisbon, Portugal; and CWHO Collaborating Centre on the Biological Characterization of Malaria Parasites, Institute of Health Research and Faculty of Science, Chulalongkorn University, Bangkok, Th...
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Genital Ureaplasma urealyticum infection is considered to be a sexually transmitted infection. The bacterium has been found to be involved in PID, chorioamnionitis, urethritis, respiratory distress syndrome and pneumonia in newborn, abortion and infertility. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. The purp...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1993
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.31.5.1345-1349.1993